| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | VASQUEZ, Heloisa | Danville | 56.14 | |
| 3 | PAYNE, Pink | Fredericktown | 55.31 | Northmor HS Quad |
| 4 | HACKMAN, Kelsey | Danville | 50.98 | Jim Proper Invitational |
| 5 | GRIFFITH, Olivia | Centerburg | 53.42 | Bob Royer Invitational |
| 6 | ALEXANDER, Jenny | Fredericktown | 54.88 | Freddie HS Quad |
| 7 | WILSON, Mackenzie | East Knox | 56.60 | Jim Proper Invitational |