| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | GREENE, Emilee | Clear Fork | 3:06.77 | Clear Fork HS 3-4-5 |
| 2 | JOHNSON, Addison | Clear Fork | 3:37.56 | Northmor HS Quad |
| 3 | BURGHOLDER, Grace | Clear Fork | 2:44.26 | 74th Marion Night Invitational |
| 4 | SHAFFNER, Ella | Galion | 3:19.17 | John McDonough Invitational |
| 5 | NEAL, Katrina | Clear Fork | 2:46.78 | Northmor HS Quad |