| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | PAYNE, Pink | Fredericktown | 55.30 | Fredericktown Invitational |
| 3 | ASHBROOK, Dixie | John. Northridge | 55.28 | Hank Smith Invitational |
| 5 | GRIFFITH, Olivia | Centerburg | 53.28 | Centerburg HS Quad |
| 6 | ALEXANDER, Jenny | Fredericktown | 54.88 | Freddie HS Quad |
| 7 | GROSS, Claire | Zanesville | 1:04.60 | Licking Valley Quad - Heath/NC/Z |
| 8 | CARDER, Sasha | Zanesville | 1:04.70 | Licking Valley Quad - Heath/NC/Z |