| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 4 | BULKOWSKI, Max | Mount Gilead MS | 35.82 | Clear Fork MS Invitational |
| 5 | FORBY, Tate | Centerburg MS | NT | |
| 6 | BLAKESLEY, Beaux | Centerburg MS | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | WALTERS, Samson | Galion Northmor JH | 32.84 | Mount Gilead MS Invitational |
| 3 | HANNAN, Reece | Fredericktown MS | 32.28 | Northmor MS Meet |
| 4 | CHAPMAN, Brady | Galion Northmor JH | 29.17 | Mount Gilead MS Invitational |
| 5 | KELLER, Aiden | Mount Gilead MS | 30.07 | Mount Gilead MS Invitational |
| 6 | PAYNE, Wyatt | Fredericktown MS | 32.26 | Redbird Invitational |
| 7 | SANDERS, Kaden | Danville Grade School | 33.21 | Race at the Lake |
| 8 | HACKMAN, Kyle | Danville Grade School | 34.41 | Race at the Lake |