| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Mount Gilead | A | Miller | Davis | Long | Bowersmith | 46.99 | |
| 3 | Northmor | A | Landin | Sippel | Amens | Stockdale | 46.56 | Buckeye Central Invitational |
| 4 | Centerburg | A | Harris | Lemaster | Mead | Turske | 44.30 | Newark Catholic Invitational |
| 5 | East Knox | A | Baker | Lamneck | Madden | Clark | 44.98 | Fredericktown Invitational |
| 6 | Fredericktown | A | Roush | Toombs | Bellman | Estep | 45.73 | Freddie HS Quad |
| 7 | Danville | A | Hackman | Meade | Proper | Mickley | 47.32 | |
| 8 | Cardington-Lincoln | A | Adams-trout | Bockbrader | Poppell | Stover | 50.62 | Buckeye Central Invitational |