| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | STREBY, Malena | East Knox | 2:44.92 | Fredericktown Invitational |
| 2 | STALEY , Kimberly | Mount Gilead | 2:33.09 | Freddie HS Quad |
| 3 | KERSHNER, Ellie | Fredericktown | 2:36.15 | KMAC HS Championship |
| 4 | HALLABRIN, Magi | Cardington-Lincoln | 2:26.50 | |
| 5 | ROOK, Kacie | Fredericktown | 2:39.24 | KMAC HS Championship |
| 6 | FURAY, Lilliana | Danville | 3:33.25 | Centerburg HS Quad |
| 7 | HILL, Claire | Centerburg | 3:00.89 | Freddie MS Quad |
| 8 | BRINKMAN, Ryann | Northmor | 2:36.82 | Mount Gilead HS Indian Invitatio |
| 9 | GARNER, Audrey | Centerburg | 2:53.63 | Bob Royer Invitational |
| 10 | PFEIFER , Haley | Mount Gilead | 2:48.37 | |
| 11 | CLUTTER, Adrian | East Knox | 2:44.29 | Jackets Invitational |
| 12 | HUNTER, Natalie | Northmor | NT |