| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Danville | A | SMITH | Shaffer | Lucas | Shields | 2:01.05 | Millersport HS Laker Invitationa |
| 3 | Centerburg | A | Nelson | Neighborgall | Moore | Richardson | 2:00.55 | Cowen Invitational |
| 4 | Mount Gilead | A | Waugh | Dillon | White | Pohlkotte | 1:53.71 | John Olin Night Invitational |
| 5 | East Knox | A | Tucker | Bostic | Streby | Fawcett | 1:54.14 | Fredericktown Invitational |
| 6 | Fredericktown | A | Feeney | Enzor | Carpenter | Scott | 1:54.36 | Fredericktown Invitational |
| 7 | Northmor | A | Barler | Castle | Erlsten | Walker | 2:02.23 | Buckeye Central Invitational |