| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | SIPES, Sunshine | St. Peter's | NT | |
| 4 | MARTIN, Caroline | Mans. Christian | 31.87 | Fredericktown Invitational |
| 5 | ENDSLOW, Natalie | Loudonville | 32.12 | Bob Royer Invitational |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | GIESIGE, Addysen | Crestline | NT | |
| 3 | NOBLIT, Jocelyn | Crestline | 30.01 | Buckeye Central Stache Dash |
| 4 | DEFRANCISCO, Lacy | Mans. Christian | 28.82 | Fredericktown Invitational |
| 5 | STONE, Alaina | Lucas | 29.96 | Shelby Track Invitational |
| 6 | ENGLAND, Maggie | Loudonville | 30.03 | Cowen Invitational |
| 7 | SMITH, Isabella | Lucas | 30.83 | Cowen Invitational |