| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | HILL, Ahzirrea | St. Peter's | 1:02.57 | Mid Buckeye Conference Champions |
| 2 | LAVENE, Lexya | Crestline | 1:02.00 | Bob Valentine Invitational - Hil |
| 3 | CASE, Leah | Lucas | 56.79 | Bob Royer Invitational |
| 4 | VERMILYA, Mya | Loudonville | 50.65 | Smithie Frosh/Soph |
| 5 | TEDROW, Jordan | Lucas | 55.82 | Bob Royer Invitational |
| 6 | HILL, Addison | Crestline | 58.10 | Bob Valentine Invitational - Hil |
| 7 | PETERSON, Brooklynn | Mans. Christian | 1:02.38 | Mount Gilead HS Indian Invitatio |