| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Mans. Christian | A | Clark | Gregory | Martin | Pond | 1:42.58 | Mount Gilead HS Indian Invitatio |
| 4 | Lucas | A | Bichsel | Guegold | Fairchild | Culler | 1:37.75 | Cowen Invitational |
| 5 | Loudonville | A | Carr | Finley | Gutierrez | Strouse | 1:42.33 | Bob Royer Invitational |
| 6 | St. Peter's | A | Hart | Lindsay | Brown | Thompson | 1:44.81 | Fredericktown Invitational |