| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | St. Peter's | A | Reef | Bond | Gilland | Wolboldt | 5:32.68 | Fredericktown Invitational |
| 3 | Mans. Christian | A | Cramer | Davis | DeFrancisco | Them | 4:48.33 | Mount Gilead HS Indian Invitatio |
| 4 | Loudonville | A | Shultz | England | Vermilya | Shultz | 4:42.92 | Cowen Invitational |
| 5 | Crestline | A | Lavene | Schmidt | Hill | Giesige | 4:46.73 | Ridgedale Track Invite |
| 6 | Lucas | A | Sturts | Shay | Tedrow | Case | 5:02.18 | Madison Comprehensive Invitation |