| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Mansfield Christian MS | A | Leach | Davis | Waters | Williamson | 1:08.13 | Mount Gilead MS Invitational |
| 4 | Loudonville JH | A | Frankford | Manchester | Brecheisen | Freelon | 55.62 | Redbird Invitational |
| 5 | St. Peter's | A | Taylor | Richlovsky | Bass | Powell | 59.62 | Plymouth Memorial Invitational |
| 6 | Lucas MS | A | Cuthbertson | McGregor | McGugin | Baker | 1:15.83 | Crestline |