| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Marion Harding | A | Ward | Tackett | Swihart | Ramirez | NT | |
| 2 | Centennial | A | NT | |||||
| 3 | Madison-Plains | A | Crites | Daulton | Riffle | Madden | NT | |
| 4 | London | A | Jackman | Mcfarlane | Neal | McQuirt | 3:47.0h | |
| 5 | Fredericktown | A | Snow | McManis | Smith | Hogg | 3:47.50 | |
| 6 | Cristo Rey | A | Residor | Crawford | Ruiz Garduno | Yorke | NT | |
| 7 | Lee. Fairfield | A | Lightle | Ayers | Burgess | Rowe | NT | |
| 8 | Amanda-Clearcreek | A | Ritchie | Lown | Moore | Williams | NT |