100,100,200,400
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Centennial | A | NT | |||||
| 2 | Lee. Fairfield | A | Lightle | Burgess | Morris | Rowe | NT | |
| 3 | Madison-Plains | A | Allgeier | Webb | Riffle | Starr | NT | |
| 4 | London | A | Graham | Cooper | Lewis | Herman | 1:41.0h | |
| 5 | Fredericktown | A | Sipes | Toombs | Estep | Snow | 1:47.50 | |
| 6 | Amanda-Clearcreek | A | Williams | Jones | Westhoven | Marshall | NT | |
| 7 | Marion Harding | A | Thrapp | Lavette | Rich | Weaver | NT | |
| 8 | Cristo Rey | A | Residor | Thomas | Yorke | Crawford | NT |