| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Mans. Ontario MS | A | Foltz | Kilgore | Secrist | Moton | NT | |
| 2 | Fredericktown MS | A | Lindeman | Kanavel | Overholt | Sharp | 1:08.39 | Northmor MS Meet |
| 3 | East Knox JH | A | Barner | Barner | Shelley | Beatty | 59.21 | Northmor MS Meet |
| 4 | Northmor JH | A | Burgess | Hedrick | Miley | Baker | 59.83 | Northmor MS Relay Invite |
| 5 | Lexington JH | A | Murfield | Woodruff | Mayer | Harwell | 1:04.16 | Mans. @ Lexington |
| 6 | Clear Fork MS | A | Dudley | Myers | Vidonish | Bieri | NT |