| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 4 | FURAY, Lilliana | Danville | 21.97 | West Holmes Quad |
| 5 | FOSTER, Lilly | Fredericktown | 22.06 | Clear Fork HS 3-4-5-6 |
| 6 | MAYFIELD, Emily | Mount Gilead | 22.74 | Jim Proper Invitational |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | PAYNE, Pink | Fredericktown | 20.14 | Clear Fork HS 3-4-5-6 |
| 3 | SAMPLES, Maycee | Danville | 20.10 | |
| 4 | HACKMAN, Kelsey | Danville | 16.8h | |
| 5 | TRAINER, Kenlee | Mount Gilead | 18.27 | John Olin Night Invitational |
| 6 | BAULT, Bailey | Mount Gilead | 19.55 | 50th Ray Mitchell Lexington Invi |
| 7 | PAYNE, Pilar | Fredericktown | 21.16 | Clear Fork HS 3-4-5-6 |