| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Lucas MS | A | Amert | Guegold | Rannigan | Hicks | NT | |
| 4 | Mount Gilead MS | A | Mosher | Eckelberry | Walsh | Grimm | 3:59.06 | Triad MS Invitational State Qual |
| 5 | Centerburg MS | A | Dickhof | Hodge | Kloss | Larimore | 4:53.26 | Mount Gilead MS Meet |
| 6 | Utica JH | A | Ulrich | Harstine | Swartzenberger | Taylor | NT |