| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Danville | A | Wallace | Mosher | Gronberg | Hackman | 4:08.66 | Jim Proper Invitational |
| 3 | New London | A | Brownell | Smith | Carruthers | Twining | 4:00.31 | NL/SC/Monroeville |
| 4 | Mount Gilead | A | Hand | Bowersmith | Stalnaker | Swalley | 3:50.00 | |
| 5 | Cardington-Lincoln | A | Brehm | McNichols | Stover | Garrison | 3:51.40 | Mike Armstrong Invitational |
| 6 | Loudonville | A | Finley | Sage | Strouse | Caley | 3:54.10 | Healthpoint Early Bird Husky Inv |
| 7 | Centerburg | A | Caudill | Jackson | Nelson | Indiciani | 4:17.47 | HS- Highland Centerburg, MCS |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Smithville | A | Geiser | Durstine | Hershey | Indermuhle | 3:48.0h | |
| 2 | Lucas | A | Bichsel | Cox | Lewis | Guegold | 3:43.84 | Col. Crawford Grove/Keller Invit |
| 3 | Fredericktown | A | Wenger | Hogg | McManis | Smith | 3:43.77 | Red Raider Relays |
| 4 | Northmor | A | Hunter | Yunker | Amens | Becker | 3:34.69 | Col. Crawford Grove/Keller Invit |
| 5 | Mar. Highland | A | Reichenbach | Rogers | Winkelfoos | Harmon | 3:38.95 | Dick Houston Relays |
| 6 | East Knox | A | Faucett | Hercenberg | Bostic | Whitney | 3:42.36 | |
| 7 | Hillsdale | A | Thomas | Burgett | Brown | Baker | 3:44.65 | Healthpoint Early Bird Husky Inv |
| 8 | Columbia | A | Skor | Johnson | Wilmeth | Washburn | NT | Clyde Hybrid Meet |