| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | PLETCHER, Will | Fredericktown | 26.47 | Clear Fork HS 3-4-5-6 |
| 2 | PAYNE, Wyatt | Fredericktown | 20.40 | Clear Fork HS 3-4-5-6 |
| 3 | HAMMITT, Dylan | Loudonville | 19.96 | 2025 Crestline Invitational |
| 4 | RINE, Cooper | Fredericktown | 15.99 | Cowen Invitational |
| 5 | HERSHBERGER, Josiah | Loudonville | 17.49 | Cowen Invitational |
| 6 | WHETNALL, Bristol | Cardington-Lincoln | 19.15 | Cowen Invitational |
| 7 | WELCH, Conor | John. Northridge | 20.72 | Johnnie Under the Lights Invitat |