| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 5 | BALLARD, Veronica | Mar. Highland MS | 5:57.90 | 2025 Bucyrus MS Invitational |
| 6 | SUDOL, Raegan | Clear Fork MS | 6:03.60 | MOAC Middle School Championship |
| 7 | MOLINA, Eliyah | Clear Fork MS | 6:33.14 | Northmor MS Meet |
| 8 | LEADBETTER, Italia | Mar. Highland MS | 6:42.65 | 2025 Bucyrus MS Invitational |
| 9 | RHEA, Makayla | Mar. Highland MS | 7:29.54 | MS - Highland , RV , and Grant |