| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Galion MS | A | Martin | Frankhouse | Hall | Stoops | 2:08.12 | 2025 Bucyrus MS Invitational |
| 3 | Clear Fork MS | A | Roberts | Wenger | Bieri | Beck | 1:57.43 | Madison Middle School Invitation |
| 4 | Mar. Highland MS | B | Rhea | Smith | Trainer | Kelley | 2:07.18 | 2025 Bucyrus MS Invitational |
| 5 | Mar. Highland MS | A | Downing | Newsome | Reichenbach | Rivers | 2:07.18 | 2025 Bucyrus MS Invitational |