| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Clear Fork MS | A | Weikle | Thrush | Freeman | Beck | 4:34.77 | Madison Middle School Invitation |
| 4 | Mar. Highland MS | A | Ballard | Green | Hedrick | Kellogg | 4:40.0h | |
| 5 | Galion MS | A | Frankhouse | Moneysmith | Kauffman | Pawsey | 5:24.45 | 2025 Bucyrus MS Invitational |