| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Cardington-Lincoln | A | Heacock | Ireland | Adams-trout | Poppell | 50.08 | Seneca East Invitational |
| 4 | Fredericktown | A | Sipes | Toombs | Rine | Estep | 44.61 | Cowen Invitational |
| 5 | Northmor | A | Stockdale | Deisch | Amens | Black | 46.03 | Col. Crawford Grove/Keller Invit |
| 6 | Lucas | A | Culler | Guegold | Payne | Ammons | 47.59 | Cowen Invitational |