| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 4 | PAYNE, Wyatt | Fredericktown | 49.53 | Clear Fork HS 3-4-5-6 |
| 5 | PAYNE, Karter | Lucas | 49.98 | Bob Royer Invitational |
| 6 | PLETCHER, Will | Fredericktown | 1:05.30 | Clear Fork HS 3-4-5-6 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | BENDER, Nolan | Fredericktown | 49.36 | Clear Fork HS 3-4-5-6 |
| 2 | BLACK, Trevor | Northmor | 49.13 | Gilead Christian High School Inv |
| 3 | WHETNALL, Bristol | Cardington-Lincoln | 47.32 | Cowen Invitational |
| 4 | RINE, Cooper | Fredericktown | 43.45 | Cowen Invitational |
| 5 | DESTERHAFT, Joey | Lucas | 43.52 | OHSAA Division 3 District - Colo |
| 6 | BREHM, AJ | Cardington-Lincoln | 43.96 | Bill Krause Memorial |
| 7 | EBERSOLE, Aidan | Fredericktown | 49.22 | Newark Catholic Invitational |