| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 4 | Northmor | A | Hunter | Stockdale | Amens | Becker | DQ | Col. Crawford Grove/Keller Invit |
| 5 | Fredericktown | A | Estep | Snow | Toombs | Sipes | 1:33.07 | Freddie HS Quad |
| 6 | Cardington-Lincoln | A | Townsend | Lawler | Boggs | Bockbrader | 1:41.37 | Seneca East Invitational |