| # | Meet | 46.78 | Caitlyn Morgan | Gilead Christian |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | WHITLATCH, Calla | Newark Cath. | NT | |
| 3 | GREEN, Rose | Mar. Highland | 1:10.41 | HS- Highland Centerburg, MCS |
| 4 | PAYNE, Pilar | Fredericktown | 58.68 | Northmor HS Quad |
| 5 | HILL, Ahzirrea | St. Peter's | 58.99 | Shelby HS Invitational |
| 6 | ELLIS, Afton | Centerburg | 1:02.49 | HS- Highland Centerburg, MCS |
| 7 | BARNER, Karoline | Newark Cath. | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | CLUTTER, Adrian | East Knox | 54.47 | Cowen Invitational |
| 2 | PRUETT, Abbie | Mar. Highland | 54.02 | |
| 3 | PAYNE, Pink | Fredericktown | 53.14 | KMAC HS Championship |
| 4 | GRIFFITH, Olivia | Centerburg | 50.22 | OHSAA Division 3 District - West |
| 5 | RICKETT, Cora | Mount Vernon | 51.95 | Jackets Invitational 2024 |
| 6 | SMITH, Charlotte | Mount Vernon | 52.90 | Licking Valley Tri Mt. Vernon He |
| 7 | YATES, Baileigh | Utica | 54.24 | Johnnie Under the Lights Invitat |
| 8 | WILSON, Mackenzie | East Knox | 56.18 | KMAC HS Championship |