| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Danville | A | Johnson | Tharp | Sanders | Railing | 1:48.0h | |
| 3 | Centerburg | B | Crane | Dickerson | Nelson | Rhodebeck | 1:45.00 | |
| 4 | East Knox | A | Bennett | Woods | Proudfoot | Beatty | 1:32.14 | Cowen Invitational |
| 5 | Fredericktown | A | Platt | Roush | Ebersole | Hogg | 1:32.50 | Fredericktown Invitational |
| 6 | Centerburg | A | Ballinger | BALL | Indiciani | Marshall | 1:38.78 | Cowen Invitational |