| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | ROUB, Alivia | Mans. Christian | 1:05.52 | Plymouth Memorial Invitational |
| 2 | GREEN, Grace | Lucas | 1:03.98 | Mount Gilead High School Invitat |
| 3 | HILL, Ahzirrea | St. Peter's | 58.26 | Mount Gilead High School Invitat |
| 4 | HILL, Addison | Crestline | 54.68 | Bob Valentine Invitational |
| 5 | RADER, Marlea | Mans. Christian | 55.99 | |
| 6 | CASE, Leah | Lucas | 56.60 | Mike Armstrong Invitational |
| 7 | SANDERS, LaVanna | Crestline | 1:05.00 |